dmem high glucose cell culture media  (Thermo Fisher)

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Reconstitution of Morphogen Signaling Gradients in Cultured Cells

doi: 10.1007/978-1-0716-1174-6_4

Figure Lengend Snippet: Engineering clonal stable sender and receiver cell lines. A) The constructs used for creating senders and receivers based on a piggyBac transposase system. B) Procedures for transfecting constructs and selecting for clonal populations. Constructs are transfected into wild-type NIH3T3 cells together with a plasmid overexpressing piggyBac transposase. Cells stably integrated with the desired plasmids are selected with antibiotics. Clonal populations with desired features (e.g. high expression level of SHH) were further sorted. C) Co-culture of senders and receivers is used for producing radial or linear gradients that can be quantitatively imaged and analyzed.

Article Snippet: NIH3T3 Cell Culture Media High glucose DMEM (Thermofisher 11960044) supplemented with the following: 10% Cosmic Calf Serum (GE Healthcare SH30087.03) 1x Penicillin-Streptomycin-Glutamine (Gibco 10378016) 1 mM sodium pyruvate (Gibco 11360070) 2.2.2.

Techniques: Construct, Transfection, Plasmid Preparation, Stable Transfection, Expressing, Co-Culture Assay

Journal: Cell reports

Article Title: Molecular motor protein KIF5C mediates structural plasticity and long-term memory by constraining local translation

doi: 10.1016/j.celrep.2021.109369

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: GIBCO DMEM, high glucose, no glutamine Cell Culture Media , Thermo Fisher , 11-960-044.

Techniques: Virus, Recombinant, Cell Culture, Saline, Sterility, Live Cell Imaging, Transfection, Protein Extraction, Protease Inhibitor, Labeling, SYBR Green Assay, Ointment, Adhesive, Gel Extraction, Isolation, TA Cloning, Sequencing, Plasmid Preparation, shRNA, Software, Control